A method of detecting abasic AP sites in DNA from a subject includes isolating a sample of DNA from a subject under examination contacting the DNA with a fluorescent aldehyde reactive probe FARP and detecting FARP labeled AP sites in the DNA sample US20090298077A1 Assay for measurement of apurinic apyrimidinic ap sites and for
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The purities of the oligonucleotides were assessed by analytical HPLC and ion spray mass spectroscopy and were found to be pure by analytical HPLC and better than 98 pure by mass spectroscopy For the abasic site and 8oxodG lesion containing oligonucleotides extinction coefficients were found to be ε CAG F n Mill Valley CA USA
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12 ensp 0183 ensp The abasic site lesion is mutagenic or lethal if not repaired From a chem point of view the abasic site is an alkali labile residue that leads to strand breakage through β and δ elimination Progress in the understanding of the chem and enzymol of abasic DNA largely relies upon the study of synthetic abasic duplexes